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KMID : 0379119850130020079
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Korean Journal of Mycology
1985 Volume.13 No. 2 p.79 ~ p.82
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Studies on Protoplast Regeneration and Reversion of Pleurotus ostreatus and Pleurotus florida
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Abstract
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The experiment of protoplast regeneration and reversion were undertaken to provide a basic techniques for protoplast manipulation. Protoplasts of Pleurotus florida and Pleurotus ostreatus were reverted to normal hyphal growth and the reversion frequency of both fungal protoplasts were 0.24∼3.19%. Reversion medium stabilized with 0.6 M potassium chloride and sucrose was better than the other stabilized one. The protoplast reversion frequency was increased when various amino acid and vitamin compounds were added to the hypertonic mushroom complete agar medium.
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